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. 2001 Oct 1;2(10):735-40.
doi: 10.1002/1439-7633(20011001)2:10<735::aid-cbic735>3.0.co;2-#.

Artificial nucleases

C B Chen  1 , L Milne, R Landgraf, D M Perrin, D S Sigman
Affiliations

Artificial nucleases

C B Chen et al. Chembiochem. .

Abstract

The oxidation of DNA and RNA provides a facile approach for investigating the interaction of nucleic acids with proteins and oligonucleotides. In this article, we have outlined our understanding of the mechanism of DNA scission by 1,10-phenanthroline-copper(I) in the presence of hydrogen peroxide. We also discuss results obtained by using 1,10-phenanthroline-oligonucleotide conjugates in probing the size of the transcriptionally active open complex. Finally, we outline an effective method for converting DNA-binding proteins into site-specific modification agents by using 1,10-phenanthroline-copper(I).

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