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. 2002 May;46(5):1269-72.
doi: 10.1128/AAC.46.5.1269-1272.2002.

Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella strains isolated in the United States between 1996 and 1998

A Carattoli  1 , F Tosini, W P Giles, M E Rupp, S H Hinrichs, F J Angulo, T J Barrett, P D Fey
Affiliations

Characterization of plasmids carrying CMY-2 from expanded-spectrum cephalosporin-resistant Salmonella strains isolated in the United States between 1996 and 1998

A Carattoli et al. Antimicrob Agents Chemother. 2002 May.

Abstract

Sequencing of DNA from 15 expanded-spectrum cephalosporin (e.g., ceftriaxone)-resistant Salmonella isolates obtained in the United States revealed that resistance to ceftriaxone in all isolates was mediated by cmy-2. Hybridization patterns revealed three plasmid structures containing cmy-2 in these 15 isolates. These data suggest that the spread of cmy-2 among Salmonella strains is occurring through mobilization of the cmy-2 gene into different plasmid backbones and consequent horizontal transfer by conjugation.

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Figures

FIG. 1.
FIG. 1.
Restriction analysis (left panels in the pairs of panels) and cmy-2 Southern hybridization (right panels in the pairs of panels). PstI-digested plasmids were extracted from E. coli C6/SS034, DH/4204, DH/3977, and C6/2039 (type A hybridization pattern) (A); E. coli C6/4255, C6/3430, and C6/1358 (type B hybridization pattern) (B); and E. coli DH/4656 (type C hybridization pattern C) (C). A 1-kb marker (KiloBase DNA marker; Pharmacia Biotech, Milan, Italy) (A and B) and a 12-kb ladder (Gibco BRL) (C) were used as standards.
See this image and copyright information in PMC

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