Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 May;46(5):1604-6.
doi: 10.1128/AAC.46.5.1604-1606.2002.

Characterization of a laboratory-derived, high-level ampicillin-resistant Salmonella enterica serovar Typhimurium strain that caused meningitis in an infant

Cheng-Hsun Chiu  1 , Chishih Chu, Lin-Hui Su, Wan-Yu Wu, Tsu-Lan Wu
Affiliations

Characterization of a laboratory-derived, high-level ampicillin-resistant Salmonella enterica serovar Typhimurium strain that caused meningitis in an infant

Cheng-Hsun Chiu et al. Antimicrob Agents Chemother. 2002 May.

Abstract

A Salmonella enterica serovar Typhimurium strain that harbored a plasmid carrying a TEM-1-type beta-lactamase gene was isolated from the blood and cerebrospinal fluid of an infant with meningitis. This 3.2-kb plasmid was further characterized to be a nonconjugative pGEM series cloning vector containing a foreign insert. The strain was likely laboratory derived and contaminated the environment before it caused the infection.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Plasmid profiles and DNA fingerprint patterns of the clinical isolate of S. enterica serovar Typhimurium and the laboratory strains. The plasmid profile was determined by the method of Kado and Liu, and the DNA fingerprints were determined by IRS-PCR. (A) Plasmid profiles. Lane 1, HB101/pSTCG; lane 2, the isolate (CGST1); lane 3, C5/pSTCG; lane 4, CNP101/pSTCG; lane 5, LBNP4417/pSTCG; lane 6, CNP101. (B) IRS-PCR patterns. Lane 1, the clinical isolate (CGST1); lane 2, CNP101; lane 3, CNP505; lane 4, LBNP4417/pSTCG; lane 5, C5; lane 6, C5/pSTCG; lane 7, HB101/pSTCG; lane M, 20-bp DNA ladder. Band sizes (in base pairs or kilobase pairs) are shown at the left.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Chiu, C. H., and J. T. Ou. 1996. Rapid identification of Salmonella serovars in feces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture-multiplex PCR combination assay. J. Clin. Microbiol. 34:2619-2622. - PMC - PubMed
    1. Chiu, C. H., T. Y. Lin, and J. T. Ou. 1999. Prevalence of the virulence plasmids of nontyphoid Salmonella in the serovars isolated from humans and their association with bacteremia. Microbiol. Immunol. 43:899-903. - PubMed
    1. Chiu, C. H., T. Y. Lin, and J. T. Ou. 1999. Predictors for extraintestinal infection of non-typhoidal Salmonella in patients without AIDS. Int. J. Clin. Pract. 53:161-164. - PubMed
    1. Chiu, C. H., T. Y. Lin, and J. T. Ou. 2000. Lack of evidence of an association between the carriage of virulence plasmid and the bacteremia of Salmonella typhimurium in humans. Microbiol. Immunol. 44:741-748. - PubMed
    1. Kado, C. I., and S. T. Liu. 1981. Rapid procedure for detection and isolation of large and small plasmids. J. Bacteriol. 145:1365-1373. - PMC - PubMed

Publication types