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. 2002 May;46(5):1604-6.
doi: 10.1128/AAC.46.5.1604-1606.2002.

Characterization of a laboratory-derived, high-level ampicillin-resistant Salmonella enterica serovar Typhimurium strain that caused meningitis in an infant

Cheng-Hsun Chiu  1 , Chishih Chu, Lin-Hui Su, Wan-Yu Wu, Tsu-Lan Wu
Affiliations

Characterization of a laboratory-derived, high-level ampicillin-resistant Salmonella enterica serovar Typhimurium strain that caused meningitis in an infant

Cheng-Hsun Chiu et al. Antimicrob Agents Chemother. 2002 May.

Abstract

A Salmonella enterica serovar Typhimurium strain that harbored a plasmid carrying a TEM-1-type beta-lactamase gene was isolated from the blood and cerebrospinal fluid of an infant with meningitis. This 3.2-kb plasmid was further characterized to be a nonconjugative pGEM series cloning vector containing a foreign insert. The strain was likely laboratory derived and contaminated the environment before it caused the infection.

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Figures

FIG. 1.
FIG. 1.
Plasmid profiles and DNA fingerprint patterns of the clinical isolate of S. enterica serovar Typhimurium and the laboratory strains. The plasmid profile was determined by the method of Kado and Liu, and the DNA fingerprints were determined by IRS-PCR. (A) Plasmid profiles. Lane 1, HB101/pSTCG; lane 2, the isolate (CGST1); lane 3, C5/pSTCG; lane 4, CNP101/pSTCG; lane 5, LBNP4417/pSTCG; lane 6, CNP101. (B) IRS-PCR patterns. Lane 1, the clinical isolate (CGST1); lane 2, CNP101; lane 3, CNP505; lane 4, LBNP4417/pSTCG; lane 5, C5; lane 6, C5/pSTCG; lane 7, HB101/pSTCG; lane M, 20-bp DNA ladder. Band sizes (in base pairs or kilobase pairs) are shown at the left.
See this image and copyright information in PMC

References

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