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. 2002 May;68(5):2595-9.
doi: 10.1128/AEM.68.5.2595-2599.2002.

Prevalence of Enterocytozoon bieneusi in swine: an 18-month survey at a slaughterhouse in Massachusetts

Michael A Buckholt  1 , John H Lee, Saul Tzipori
Affiliations

Prevalence of Enterocytozoon bieneusi in swine: an 18-month survey at a slaughterhouse in Massachusetts

Michael A Buckholt et al. Appl Environ Microbiol. 2002 May.

Abstract

Slaughterhouse pig samples were analyzed by PCR for Enterocytozoon bieneusi infection. Thirty-two percent were found to be positive, with rates being higher over the summer months. Three isolates from pigs were identical in their ribosomal internal transcribed spacer sequence to human E. bieneusi type D, two were identical to type F (from a pig), and nine were previously unreported. The viability of these spores was demonstrated by their ability to infect gnotobiotic piglets. The presence of the infection in liver was shown by in situ hybridization.

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Figures

FIG. 1.
FIG. 1.
Dendrogram of E. bieneusi genotypes. The plot was constructed by the Jukes-Cantor correction method, modified to consider gaps. Distances are estimated numbers of substitutions per 100 bases, as represented by the bar. Genotypes, hosts, and references are shown. All accession numbers are as given in footnote a of Table 2.
FIG. 2.
FIG. 2.
In situ hybridization of liver tissue from a slaughterhouse pig bile sample positive for E. bieneusi. Black granular staining shows a positive hybridization.
See this image and copyright information in PMC

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