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Review
. 2002 May;40(5):1575-80.
doi: 10.1128/JCM.40.5.1575-1580.2002.

Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology

B Cherie Millar  1 , Jiru Xu, John E Moore
Affiliations
Review

Risk assessment models and contamination management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology

B Cherie Millar et al. J Clin Microbiol. 2002 May.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Example of a flow chart detailing the manipulations, the contamination hazards and risks, and the corrective action involved for broad-range 16S rDNA PCR detection (in peripheral blood) of bacterial causal agents of infectious diseases. Blood-EDTA vials are blood collection vials containing EDTA. dNTPs, deoxyribonucleoside triphosphates; MLSO, medical laboratory scientific officer.
FIG. 2.
FIG. 2.
Proposed layout of laboratory space associated with broad-range 16S rDNA PCR for detection of bacterial causal agents of infectious diseases. RFLP, restriction fragment length polymorphism; SSCP, single-stranded conformational polymorphism.
See this image and copyright information in PMC

Comment in

References

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