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. 2002 May;40(5):1709-14.
doi: 10.1128/JCM.40.5.1709-1714.2002.

Detection, subgroup specificity, and genotype diversity of rotavirus strains in children with acute diarrhea in Paraguay

Norma Coluchi  1 , Veridiana Munford, Julio Manzur, Cynthia Vazquez, Mabel Escobar, Ernesto Weber, Perla Mármol, Maria Lucia Rácz
Affiliations

Detection, subgroup specificity, and genotype diversity of rotavirus strains in children with acute diarrhea in Paraguay

Norma Coluchi et al. J Clin Microbiol. 2002 May.

Abstract

Of a total of 220 stool specimens from children with acute diarrhea, mostly under the age of 3 years, collected in Paraguay between January 1999 and March 2000, 70 (31.8%) were found positive for rotaviruses (RV). Positive samples were characterized by electropherotyping and subgrouping. Sixty-one (87.1%) were classified as group A, subgroup II; one (1.4%) was classified as group A, subgroup I; six (8.6%) were group A, non-I non-II; and two (2.9%) were not tested. RV strains were G and P genotyped by reverse transcription-PCR. The following G types were detected: G4 (34.3%), G1 (21.4%), G2 (1.4%), and G9 (5.7%). Mixtures of human and animal genotypes were detected in 15 (21.4%) samples, and 11 samples (15.7%) were nontypeable. The following P types were detected: P[8] (48.6%), P[4] (1.4%), and P[1] (1.4%). A mixed type was found in 10% of samples, and an unexpectedly high percentage (38.6%) of nontypeable samples was found. The common human G- and P-type combinations P[8], G4 (15.7%) and P[8], G1 (14.2%) were detected. Mixed human and animal genotypes were observed as the following combinations: G4 + G5, G4 + G5 + G10, and G1 + G10 for G types and P[8]-P[1] for P types. The emerging G9 genotype was detected in four samples. These results show for the first time the diversity of RV circulating among children in Paraguay and contribute to the knowledge of this pathogen required to devise strategies to prevent diarrheal illness in this country. The finding of mixed genotypes may indicate interspecies transmission of RV between humans and animals.

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Figures

FIG. 1.
FIG. 1.
Total number of fecal samples, number of rotavirus-positive samples, average minimum temperature, and relative humidity by month of sample collection. All samples were collected in Paraguay (1999 to 2000).
FIG. 2.
FIG. 2.
Distribution of rotavirus G genotypes by month as determined by RT-PCR genotyping. Samples were collected in Paraguay (1999 to 2000).
See this image and copyright information in PMC

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