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. 2002 May;40(5):1826-30.
doi: 10.1128/JCM.40.5.1826-1830.2002.

Sequence analysis of the ribosomal DNA intergenic spacer 1 regions of Trichosporon species

Takashi Sugita  1 , Masamitsu Nakajima, Reiko Ikeda, Toshiharu Matsushima, Takako Shinoda
Affiliations

Sequence analysis of the ribosomal DNA intergenic spacer 1 regions of Trichosporon species

Takashi Sugita et al. J Clin Microbiol. 2002 May.

Abstract

We determined the sequence of the intergenic spacer (IGS) 1 region, which is located between the 26S and 5S rRNA genes, in 25 species of the genus TRICHOSPORON: IGS 1 sequences varied in length from 195 to 719 bp. Comparative sequence analysis suggested that the divergence of IGS 1 sequences has been greater than that of the internal transcribed spacer regions. We also identified five genotypes of T. asahii, which is a major causative agent of deep-seated trichosporonosis, based on the IGS 1 sequences of 43 strains. Most of the isolates that originated in Japan were of genotype 1, whereas the American isolates were of genotype 3 or 5. Our results suggest that analysis of IGS regions provides a powerful method to distinguish between phylogenetically closely related species and that a geographic substructure may exist among T. asahii clinical isolates.

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Figures

FIG. 1.
FIG. 1.
Schematic representation of the rDNA locus in Trichosporon. Boxes indicate coding regions.
FIG. 2.
FIG. 2.
Sequence similarities between IGS 1 and ITS regions “% ITS sequence similarity” indicates similarity between combined ITS 1 and ITS 2 sequences.
FIG. 3.
FIG. 3.
A phylogenetic tree of five IGS 1 genotypes of T. asahii. The sequences were aligned using CLUSTAL W (version 1.8) software (18), and the tree was constructed using TreeView (version 1.6.2) (http://taxonomy.zoology.gla.ac.uk/rod/treeview.html).
See this image and copyright information in PMC

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