Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis
- PMID: 11980982
- PMCID: PMC130930
- DOI: 10.1128/JCM.40.5.1873-1874.2002
Tetrazolium microplate assay as a rapid and inexpensive colorimetric method for determination of antibiotic susceptibility of Mycobacterium tuberculosis
Abstract
The emergence of multidrug-resistant tuberculosis underscores the need for low-cost, rapid methods to determine the susceptibility of Mycobacterium tuberculosis to antibiotics. A new, rapid, easily read, and inexpensive colorimetric method with a tetrazolium indicator performs this determination as quickly and accurately as the more expensive Alamar Blue technique.
References
-
- Franzblau, S. G., R. S. Witzig, J. C. McLaughlin, P. Torres, G. Madico, A. Hernandez, M. T. Degnan, M. B. Cook, V. K. Quenzer, R. M. Ferguson, and R. H. Gilman. 1998. Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay. J. Clin. Microbiol. 36:362-366. - PMC - PubMed
-
- Havlir, D. V., and P. F. Barnes. 1999. Tuberculosis in patients with human immunodeficiency virus infection. N. Engl. J. Med. 340:367-373. - PubMed
-
- Msamanga, G. I., and W. W. Fawzi. 1997. The double burden of HIV infection and tuberculosis in sub-Saharan Africa. N. Engl. J. Med. 337:849-851. - PubMed
-
- World Health Organization. 2001. Global tuberculosis control. WHO Report 2001. WHO/CDS/TB/2001.287. World Health Organization, Geneva, Switzerland.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
