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Comparative Study
. 2002 Jun;184(11):3122-5.
doi: 10.1128/JB.184.11.3122-3125.2002.

Examination of the Borrelia burgdorferi transcriptome in Ixodes scapularis during feeding

Sukanya Narasimhan  1 , Felix Santiago, Raymond A Koski, Brandon Brei, John F Anderson, Durland Fish, Erol Fikrig
Affiliations
Comparative Study

Examination of the Borrelia burgdorferi transcriptome in Ixodes scapularis during feeding

Sukanya Narasimhan et al. J Bacteriol. 2002 Jun.

Abstract

Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasmic proteins were preferentially expressed in engorging ticks. These data demonstrate a new approach to the global analysis of B. burgdorferi genes that are preferentially expressed within the vector during feeding.

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Figures

FIG. 1.
FIG. 1.
Southern analysis of the individual genes carried by the differentially hybridizing B. burgdorferi clones. The genes in selected differentially hybridizing clones were PCR amplified by use of gene-specific primers. The PCR products were Southern blotted to nitrocellulose membranes. Shown are Southern blots hybridized to the radioactively labeled Fed Bb-DECAL probe (A and B) and to the radioactively labeled Unfed Bb-DECAL probe (C).
FIG. 2.
FIG. 2.
Confirmation of DECAL and differential immunoscreening results by RT-PCR. cDNA prepared from B. burgdorferi-infected unfed and fed I. scapularis nymphs were used as templates in PCRs with gene-specific primers.
See this image and copyright information in PMC

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