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. 1975 Nov-Dec;11(6):395-9.
doi: 10.1007/BF02616376.

Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L)

P E Eide, J M Caldwell, E P Marks

Establishment of two cell lines from embryonic tissue of the tobacco hornworm, Manduca sexta (L)

P E Eide et al. In Vitro. 1975 Nov-Dec.

Abstract

Two cell lines were derived from primary cultures of embryonic tissue of the tobacco hornworm, Manduca sexta (L.). The cell lines were maintained on hemolymph-free synthetic insect medium. Cytogenetic and immunoligical identification of the lines were carried out. Techniques for obtaining the line and medium, subculturieng and freezing procedures for long-term storage, and the morphological and growth characteristics are described.

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