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. 2002 Jun;46(6):2004-6.
doi: 10.1128/AAC.46.6.2004-2006.2002.

Chromosome-encoded class D beta-lactamase OXA-23 in Proteus mirabilis

R Bonnet  1 , H Marchandin, C Chanal, D Sirot, R Labia, C De Champs, E Jumas-Bilak, J Sirot
Affiliations

Chromosome-encoded class D beta-lactamase OXA-23 in Proteus mirabilis

R Bonnet et al. Antimicrob Agents Chemother. 2002 Jun.

Abstract

Ten nonrepetitive Proteus mirabilis isolates, which were collected over 4 years (1996 to 1999) at the teaching hospital of Clermont-Ferrand, France, produced class D carbapenemase OXA-23. MICs of imipenem were 0.25 to 0.5 microg/ml for these clinical isolates. Molecular typing revealed that the 10 P. mirabilis isolates originated from the same clonal strain. Hybridization of I-CeuI-generated chromosome fragments with a bla(OXA-23) probe showed that the gene was chromosome encoded in the P. mirabilis strain.

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Figures

FIG. 1.
FIG. 1.
Molecular typing of P. mirabilis isolates. (A) PFGE of NotI macrorestriction fragments. (B) Ribotyping. Lanes: 1 to 3, non-OXA-23-producing P. mirabilis strains; 4 to 13, OXA-23-producing P. mirabilis isolates.
FIG. 2.
FIG. 2.
Localization of blaOXA-23 in I-Ceu I-generated chromosome fragments of P. mirabilis CFO239 separated by PFGE. (A) Chromosome restriction patterns. (B) Hybridization of restricted patterns with a probe specific to 23S rRNA genes. (C) Hybridization of restricted patterns with a probe specific to the blaOXA-23 gene. Lanes: 1, clinical P. mirabilis without the blaOXA-23 gene; 2, OXA-23-producing P. mirabilis CFO239.
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References

    1. Afzal-Shah, M., H. E. Villar, and D. M. Livermore. 1999. Biochemical characteristics of a carbapenemase from an Acinetobacter baumannii isolate collected in Buenos Aires, Argentina. J. Antimicrob. Chemother. 43:127-131. - PubMed
    1. Afzal-Shah, M., N. Woodford, and D. M. Livermore. 2001. Characterization of OXA-25, OXA-26, and OXA-27, molecular class D β-lactamases associated with carbapenem resistance in clinical isolates of Acinetobacter baumannii. Antimicrob. Agents Chemother. 45:583-588. - PMC - PubMed
    1. Ambler, R. P., A. F. W. Coulson, J.-M. Frère, J.-M. Ghuysen, B. Joris, M. Forsman, R. C. Levesque, G. Tiraby, and S. G. Waley. 1991. A standard numbering scheme for the class A β-lactamases. Biochem. J. 276:269-272. - PMC - PubMed
    1. Bingen, E., C. Boissinot, P. Desjardins, H. Cave, N. Brahimi, N. Lambert-Zechovsky, E. Denamur, P. Blot, and J. Elion. 1993. Arbitrarily primed polymerase chain reaction provides rapid differentiation of Proteus mirabilis isolates from a pediatric hospital. J. Clin. Microbiol. 31:1055-1059. - PMC - PubMed
    1. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523. - PMC - PubMed

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