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. 2002 Jun;46(6):2014-6.
doi: 10.1128/AAC.46.6.2014-2016.2002.

Detection of a variant metallo-beta-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an alcaligenes xylosoxidans strain

Shizuko Iyobe  1 , Haruko Kusadokoro, Ayako Takahashi, Sachie Yomoda, Toyoji Okubo, Akio Nakamura, Koji O'Hara
Affiliations

Detection of a variant metallo-beta-lactamase, IMP-10, from two unrelated strains of Pseudomonas aeruginosa and an alcaligenes xylosoxidans strain

Shizuko Iyobe et al. Antimicrob Agents Chemother. 2002 Jun.

Abstract

The gene bla(IMP-10) of a variant metallo-beta-lactamase, IMP-10, had a single base replacement of G by T at nucleotide 145, which led to an amino acid alteration of Val49 to Phe compared to the IMP-1 enzyme, indicating that IMP-10 was a point mutation derivative of IMP-1. Highly purified enzymes revealed that IMP-10 was different from IMP-1 in its extremely low hydrolyzing activities for penicillins, such as benzylpenicillin, ampicillin, and piperacillin.

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