Initiation and growth of microtubules from mitotic centers in lysed mammalian cells
- PMID: 1202022
- PMCID: PMC2111672
- DOI: 10.1083/jcb.67.3.744
Initiation and growth of microtubules from mitotic centers in lysed mammalian cells
Abstract
Metaphase PtK1 cells, lysed into polymerization-competent microtubule protein, maintain a spindle which will gain or lose birefringence depending on the concentration of disassembled tubulin subunits used in the lysis medium. Concentrations of tubulin subunits greater than the equilibrium monomer value promote a rate and extent of birefringence increase that is proportional to the subunit concentration. Increase in spindle birefringence can be correlated with an increase in tubule number, though the relationship is not strictly linear. Increase in spindle tubule number is due to an vivo-like initiation of tubules at the mitotic centers, as well as tubulin addition onto pre-existing spindle fragments. Colcemid-treated prometaphase cells lysed into polymerization-competent tubulin develop large asters in the region of the centrioles and short tubules at kinetochores, making it unlikely that all microtubule formation in lysed cell preparations is dependent on tubulin addition to short tubule fragments. Asters can also form in colcemid-treated prometaphase cells lysed in tubulin that is incapable of spontaneous tubule initiation, suggesting that the centriolar region serves a tubule-initiator function in our lysed cell preparations. The ability of the centriole to initiate microtubule assembly is a time-dependent process-a ripening effect takes place between prophase and late prometaphase. Ripening is expressed by an increase in the number and length of tubules found associated with the centriolar region.
Similar articles
-
Microtubule initiation at kinetochores and centrosomes in lysed mitotic cells. Inhibition of site-specific nucleation by tubulin antibody.J Cell Biol. 1979 Aug;82(2):585-91. doi: 10.1083/jcb.82.2.585. J Cell Biol. 1979. PMID: 479317 Free PMC article.
-
Human chromosomes and centrioles as nucleating sites for the in vitro assembly of microtubules from bovine brain tubulin.J Cell Biol. 1975 Oct;67(1):189-99. doi: 10.1083/jcb.67.1.189. J Cell Biol. 1975. PMID: 809450 Free PMC article.
-
Rapid rate of tubulin dissociation from microtubules in the mitotic spindle in vivo measured by blocking polymerization with colchicine.J Cell Biol. 1984 Sep;99(3):1066-75. doi: 10.1083/jcb.99.3.1066. J Cell Biol. 1984. PMID: 6470037 Free PMC article.
-
Elongation of centriolar microtubule triplets contributes to the formation of the mitotic spindle in gamma-tubulin-depleted cells.J Cell Sci. 2004 Nov 1;117(Pt 23):5497-507. doi: 10.1242/jcs.01401. Epub 2004 Oct 12. J Cell Sci. 2004. PMID: 15479719
-
Dynamics of mitotic spindle organization and function.Soc Gen Physiol Ser. 1975;30:3-30. Soc Gen Physiol Ser. 1975. PMID: 1103302 Review. No abstract available.
Cited by
-
Characteristics of the polar assembly and disassembly of microtubules observed in vitro by darkfield light microscopy.J Cell Biol. 1979 Oct;83(1):205-17. doi: 10.1083/jcb.83.1.205. J Cell Biol. 1979. PMID: 511939 Free PMC article.
-
The spindle pole body of Schizosaccharomyces pombe enters and leaves the nuclear envelope as the cell cycle proceeds.Mol Biol Cell. 1997 Aug;8(8):1461-79. doi: 10.1091/mbc.8.8.1461. Mol Biol Cell. 1997. PMID: 9285819 Free PMC article.
-
Strongylocentrotus purpuratus spindle tubulin. I. Characteristics of its polymerization and depolymerization in vitro.J Cell Biol. 1982 Jun;93(3):788-96. doi: 10.1083/jcb.93.3.788. J Cell Biol. 1982. PMID: 7119002 Free PMC article.
-
Identification of centrosomal proteins in a human lymphoblastic cell line.EMBO J. 1986 Oct;5(10):2545-50. doi: 10.1002/j.1460-2075.1986.tb04533.x. EMBO J. 1986. PMID: 3536479 Free PMC article.
-
Metaphase Spindle Assembly.Biology (Basel). 2017 Feb 3;6(1):8. doi: 10.3390/biology6010008. Biology (Basel). 2017. PMID: 28165376 Free PMC article. Review.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous