doi: 10.1016/s0021-9673(00)89019-6.
The determination of hydralazine in plasma by gas-liquid chromatography
- PMID: 1202076
- DOI: 10.1016/s0021-9673(00)89019-6
Item in Clipboard
The determination of hydralazine in plasma by gas-liquid chromatography
J Chromatogr.
.
Abstract
A description is given of a gas chromatographic method for the determination of hydralazine in plasma. On treatment with nitrous acid, hydralazine is converted into tetrazolo[1,5-a]phthalazine, a stable compound that can be extracted from biological material with organic solvent and determined quantitiatively by gas-liquid chromatography. The 4-methyl analogue of hydralazine serves as internal standard for derivatization, extraction and gas chromatography. The sensitivity (10 ng per ml of plasma) is sufficient to monitor plasma levels in man after administration of single oral doses of 50 mg of Apresoline¿. The known metabolites do not interfere.
Similar articles
-
Determination of unchanged hydralazine in plasma by gas-liquid chromatography using nitrogen-specific detection.J Chromatogr. 1979 Sep 1;176(3):375-80. doi: 10.1016/s0021-9673(00)89455-8. J Chromatogr. 1979. PMID: 546921
-
Gas chromatographic method for the simultaneous determination of hydralazine and its acetylated metabolite in serum using a nitrogen-selective detector.J Chromatogr. 1980 Aug 8;183(2):159-66. doi: 10.1016/s0378-4347(00)81689-8. J Chromatogr. 1980. PMID: 7400274
-
Selective high-performance liquid chromatographic assays for hydralazine and its metabolites in plasma of man.J Chromatogr. 1980 Mar 14;181(3-4):427-40. doi: 10.1016/s0378-4347(00)81145-7. J Chromatogr. 1980. PMID: 7391156
-
Kinetics of hydralazine elimination.Clin Pharmacol Ther. 1977 Jun;21(6):715-20. doi: 10.1002/cpt1977216715. Clin Pharmacol Ther. 1977. PMID: 862310
-
Interference in assays for hydralazine in humans by a major plasma metabolite, hydralazine pyruvic acid hydrazone.J Pharm Sci. 1978 Aug;67(8):1150-3. doi: 10.1002/jps.2600670833. J Pharm Sci. 1978. PMID: 27629
Cited by
-
High-performance liquid chromatography-tandem mass spectrometry in the identification and determination of phase I and phase II drug metabolites.Anal Bioanal Chem. 2008 May;391(1):59-78. doi: 10.1007/s00216-008-1962-7. Epub 2008 Mar 15. Anal Bioanal Chem. 2008. PMID: 18345532 Free PMC article. Review.
-
Pharmacokinetics of oral hydralazine in chronic heart failure.Eur J Clin Pharmacol. 1983;25(4):467-73. doi: 10.1007/BF00542113. Eur J Clin Pharmacol. 1983. PMID: 6653641
-
Studies on hydralazine. III. Bioavailability of hydralazine in man.Eur J Clin Pharmacol. 1976;10(6):395-401. doi: 10.1007/BF00563075. Eur J Clin Pharmacol. 1976. PMID: 1001354
-
Clinical pharmacokinetics of hydralazine.Clin Pharmacokinet. 1982 May-Jun;7(3):185-205. doi: 10.2165/00003088-198207030-00001. Clin Pharmacokinet. 1982. PMID: 7047041 Review. No abstract available.
-
Pharmacokinetics of bendroflumethiazide alone and in combination with propranolol and hydralazine.Eur J Clin Pharmacol. 1982;21(4):315-23. doi: 10.1007/BF00637620. Eur J Clin Pharmacol. 1982. PMID: 7056277