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. 1975 Dec;16(6):1375-9.
doi: 10.1128/JVI.16.6.1375-1379.1975.

Alkali lability of bacteriophage phi W-14 DNA

H A Lewis, R C Miller Jr, J C Stone, R A Warren

Alkali lability of bacteriophage phi W-14 DNA

H A Lewis et al. J Virol. 1975 Dec.

Abstract

The molecular weight of bacteriophage phi W-14 DNA, determined by velocity sedimentation in neutral sucrose gradients, was 92 +/- 6 X 10(6). The DNA showed marked fragmentation in alkaline sucrose gradients. This fragmentation was not a consequence of preexisting single-strand interruptions in the DNA, since thermal denaturation of DNA yielded intact single strands. The alpha-putrescinylthymine groups in phi W-14 DNA appeared to be labile; some, or parts of some, of these groups were cleaved from the DNA in alkali.

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References

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    1. J Mol Biol. 1973 Apr 5;75(2):213-34 - PubMed
    1. J Virol. 1974 Nov;14(5):1040-8 - PubMed
    1. Nature. 1973 Mar 30;242(5396):327-9 - PubMed
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