[Photochemical and spectral properties of photosystem-2 subchloroplast fragments, highly purified from photosystem-1 mixtures]

Abstract

A rather simple method of isolation of photosystem 2 fragments, which are highly purified from Photosystem 1 admixture, has been developed on the basis of combined action of detergents and differential centrifugation. The isolated fragments are characterized by insignificant content of P700 (one molecule per 10500 molecules of chlorophyll) and by high ratio of band values at 685 and 735 nm in the low temperature emission spectrum of fluorescence (F685/F735=5.9). The data on photochemical activity and ability for photoinduced changes in fluorescence prove that the activity of Photosystem 2 is retained both at the level of reaction centre operation and at that of water photooxidation with oxygen evolution.