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. 2002 Jun;40(6):2108-14.
doi: 10.1128/JCM.40.6.2108-2114.2002.

Genotyping study of Scedosporium apiospermum isolates from patients with cystic fibrosis

Alain Defontaine  1 , Rachid Zouhair, Bernard Cimon, Jacqueline Carrère, Eric Bailly, Françoise Symoens, Mohammed Diouri, Jean-Noel Hallet, Jean-Philippe Bouchara
Affiliations

Genotyping study of Scedosporium apiospermum isolates from patients with cystic fibrosis

Alain Defontaine et al. J Clin Microbiol. 2002 Jun.

Abstract

Usually a saprophyte, Scedosporium apiospermum often colonizes the respiratory tracts of patients with cystic fibrosis (CF). In order to improve our understanding of the molecular epidemiology of the airway colonization, 129 sequential and multiple isolates collected from January 1998 to March 1999 from nine CF patients monitored in three hospitals in France were typed by random amplification of polymorphic DNA with primers GC70, UBC-701, and UBC-703. Among these primers, UBC-703 was the most discriminating, allowing the differentiation of 14 genotypes. Combining the results obtained with this three-primer set resulted in the differentiation of 16 genotypes. No common genotype was found among the different patients, and no clustering according to geographic origin of the isolates was seen. In addition, five of the patients were colonized by a single genotype. The others usually exhibited a predominant genotype accompanied by one or two others, which were found occasionally and were genetically close to the predominant genotype. Thus, our study demonstrates the persistence of the fungus despite antifungal treatments and therefore reinforces the need for the development of new antifungals that are more efficient against this species.

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Figures

FIG. 1.
FIG. 1.
Electrophoretic patterns of the 16 genotypes identified in S. apiospermum isolates from CF patients. Amplicons generated by primers GC70 (A), UBC-701 (B), and UBC-703 (C) were separated on 1.5% agarose gels. Lanes M, DNA size markers (100-bp ladder from Amersham Pharmacia-Biotech).
FIG. 2.
FIG. 2.
Electrophoretic patterns of S. apiospermum isolates from CF patients P4 (A), P8 (B), and P9 (C). Amplicons generated by primer UBC-703 were separated on 1.5% agarose gels. Lanes M, DNA size markers (100-bp ladder from Amersham Pharmacia-Biotech).
FIG. 3.
FIG. 3.
Dendrogram constructed from RAPD data showing the genetic relationships between the 16 genotypes identified in S. apiospermum isolates from CF patients. The phylogenetic tree was generated from a similarity matrix based on the Jacquard coefficient by using UPGMA cluster analysis. The scale at the top of the figure shows the SD.
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References

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