Common protective antigen between Pseudomanas aeruginosa and Vibrio cholerae

Abstract

Immunization with a common antigen (OEP) of Pseudomonas aeruginosa produced a high level of protection in mice against Vibrio cholerae infection. The average OEP-HA titer in mice sera of immune group was 1,600 HA titer. However, vibriocidal titer and agglutinin titer against V. cholerae were not observed in sera of mice immunized with OEP. By passive administration of anti-OEP rabbit serum, mice were also protected against challenge with V. cholerae. In order to confirm the presence of the common antigen of P. aeruginosa in V. cholerae, IF-30 (common antigen of V. cholerae) was provided. Antibodies were found by the OEP-passive hemagglutination test to cross-react with the common antigen (OEP) of P. aeruginosa in sera of rabbits immunized with IF-30. The titer of OEP antibody formation in rabbits immunized with IF-30 was almost the same as that of P. aeruginosa. When mice were immunized with IF-30 and challenged with each of 7 kinds of Fisher's immunotype of P. aeruginosa, protection was observed in all the mice vaccinated against the challenge with each of the 7 kinds of strains. Furthermore, it was also confirmed that there was significant protection in mice injected with anti-IF-30 rabbit serum against all strains of Fisher's immunotype of P. aeruginosa.