Development of intertectal neuronal connections in xenopus: the effects of contralateral transposition of the eye and of eye removal

Abstract

The development of intertectal neruonal connections has been investigated in Xenopus laevis. Contralateral eye grafts and enucleations were performed in embryos and the resultant visual projections to the optic tecta were mapped electrophysiologically after metamorphosis. In enucleated animals the ipsilateral projections were found to be normally organised retinotopically but consisted of visual units with abnormally large multi-unit receptive fields. In 10 animals with contralaterally grafted eyes a normal ipsilateral projection had developed from the abnormal eye and an abnormal projection from the normal eye, to produce congruent maps via the two eyes to one tectum. All the maps in these animals were retinotopically organised. In another 11 animals the ipsilateral projection from the operated eye was fragmentary or absent, while that from the unoperated eye resembled the pattern found after enucleation. Retinotopically abnormal contralateral projections had developed in 5 animals of this group. These results suggest that prefunctional specification determines the initial development of diffuse intertectal visual connections but these may be modified by a process of binocular interaction in the presence of a normal primary contralateral input.