Functional genomics as applied to mapping transcription regulatory networks

Abstract

The sequencing of the human genome and the entire genomes of many model organisms has resulted in the identification of many genes. Many large-scale experiments for generating gene disruptions and analyzing the phenotypes are underway to ascertain gene function. A future challenge will be to determine interaction and regulation of all the genes of an organism. Recent advances in functional genomic technology have begun to shine light on such gene network problems at both transcriptomic and proteomic levels. Functional genomics will not only elucidate what the genes do, but will also help determine when, where and how they are expressed as an orchestrated system. In this review, we discuss the functional genomics approaches to extract knowledge about transcription regulatory mechanisms from combinations of sequence data, microarray data and ChIP data. We focus in particular on the budding yeast Saccharomyces cerevisiae.