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. 2002 Jul;83(1):37-42.
doi: 10.1016/s0001-706x(02)00054-2.

In vitro cultivation and characterization of Leishmania chagasi amastigote-like forms

Ashwini Somanna  1 , Vasanthakrishna Mundodi, Lashitew Gedamu
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In vitro cultivation and characterization of Leishmania chagasi amastigote-like forms

Ashwini Somanna et al. Acta Trop. 2002 Jul.

Abstract

For the first time, we have reported the establishment and serial propagation of an axenic culture of Leishmania chagasi amastigote-like forms. Parasites were characterized by microscopic evaluation and by the expression of two stage-specific genes, A2 and Ldccys2 amastigote-specific cysteine protease. The differentiated amastigote-like forms were maintained by serial cultivation.

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