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. 1975 Jan;17(1):287-90.
doi: 10.1128/JVI.17.1.287-290.1976.

Rapid purification of 70S RNA from media of cells producing RNA tumor viruses

R G Smith, L Donehower, R C Gallo, D H Gillespie

Rapid purification of 70S RNA from media of cells producing RNA tumor viruses

R G Smith et al. J Virol. 1975 Jan.

Abstract

Media from cells producing RNA tumor viruses, when treated with sodium dodecyl sulfate and polyvinyl sulfate, yield 70S RNA as the major species binding oligo(dT)-cellulose. The procedure described for purifying 70S RNA requires no special equipment and is suitable for rapidly processing large quantities of media or for purifying RNA from commercially avialable virus, with a 5- to 10-fold higher yield than was obtained using existing methods.

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