Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Jul;46(7):2303-6.
doi: 10.1128/AAC.46.7.2303-2306.2002.

blaCTX-M-2 is located in an unusual class 1 integron (In35) which includes Orf513

Sonia M Arduino  1 , Paul H Roy, George A Jacoby, Betina E Orman, Silvia A Pineiro, Daniela Centron
Affiliations

blaCTX-M-2 is located in an unusual class 1 integron (In35) which includes Orf513

Sonia M Arduino et al. Antimicrob Agents Chemother. 2002 Jul.

Abstract

Examination of the bla(CTX-M-2) gene in plasmid pMAR-12 by sequencing and PCR analysis revealed that the bla gene and the surrounding DNA, which is closely related (99% homology) to the Kluyvera ascorbata chromosomal DNA that contains the bla(KLUA-1) gene, are located in a complex sul1-type integron, termed In35, that includes Orf513. It is possible that bla(CTX-M-2) was acquired by plasmid pMAR-12 through an uncharacterized recombinational event in which Orf513 could be involved.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Structure of the integron of pMAR-12. (A) In35 containing the blaCTX-M-2 gene obtained by sequencing pMAR-121 (2,100 bp) (region I) and PCR mapping of the surrounding regions (regions II and III); (B) lengths of PCR products; (C) sequence reported here (GenBank accession number AY079169).
FIG. 2.
FIG. 2.
Comparison of the genetic structures of In6 (catII), In7(dfrA10), In35 (blaCTX-M-2), pSAL-1(blaDHA-1), and In60 (blaCTX-M-9). Sequences indicated by shaded boxes are identical. (A) A 28-bp segment at the end of the common region; (B) beginning of the common region of In6 and In7 (nucleotides in bold) that lies 24 bp beyond the stop codon of the sul1 gene (underlined) in the first 3′-CS; (C) open reading frame (orf) that is 71.6% identical to Orf3 from K. ascorbata (accession number AJ272538).
FIG. 3.
FIG. 3.
Deletions at the 5′ end of the second 3′-CS in In60 (blaCTX-M-9), In35 (blaCTX-M-2), In7 (dfrA10), pSAL-1 (blaDHA-1), and In6 (catII). Base 1 corresponds to the first base of the 3′-CS.
See this image and copyright information in PMC

References

    1. Aubert, D., L. Poirel, J. Chevalier, S. Leotard, J.-M. Pages, and P. Nordmann. 2001. Oxacillinase-mediated resistance to cefepime and susceptibility to ceftazidime in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 45:1615-1620. - PMC - PubMed
    1. Bauernfeind, A., I. Stemplinger, R. Jungwirth, R. Wilhelm, and Y. Chong. 1996. Comparative characterization of the cephamycinase blaCMY-1 gene and its relationship with other β-lactamase genes. Antimicrob. Agents Chemother. 40:1926-1930. - PMC - PubMed
    1. Bauernfeind, A., T. Stemplinger, R. Jungwirth, S. Ernst, and J. M. Casellas. 1996. Sequences of β-lactamase genes encoding CTX-M-1 (MEN-1) and CTX-M-2 and relationship of their amino acid sequences with those of other β-lactamases. Antimicrob. Agents Chemother. 40:509-513. - PMC - PubMed
    1. Bonnet, R., C. Dutour, J. L. M. Sampaio, C. Chanal, D. Sirot, R. Labia, C. De Champs, and J. Sirot. 2001. Novel cefotaximase (CTX-M-16) with increased catalytic efficiency due to substitution Asp-240→Gly. Antimicrob. Agents Chemother. 45:2269-2275. - PMC - PubMed
    1. Bush, K., G. A. Jacoby, and A. Medeiros. 1995. A functional classification scheme for β-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother. 39:1211-1233. - PMC - PubMed

Publication types

Associated data

LinkOut - more resources