Polysomes and initiation complexes in vitro induced by Semliki Forest virus 42S and 26S RNA

Abstract

Semliki Forest virus 42S and 26S RNA induce the formation of polysomes when translated in vitro in cell-free systems. After analysis on sucrose gradients the polysomal structures, containing prelabelled RNA and nascent peptide chains labelled with [35S]methionine, had sedimentation values from 100 to 200S in the case of 26S RNA and from 150 to over 250S with 42S RNA. After incubation in the presence of inhibitors of elongation 26S RNA was found in initiation complexes sedimenting at about 80S. Under identical conditions 42S RNA had a heterogenous sedimentation pattern, being attached to complexes sedimenting even faster than 250S. These structures, however, fulfilled the criteria of initiation complexes suggesting that there were more than one ribosome attached to each 42S RNA under conditions where 26S RNA bound only one.