[Separate measurement of fluoroanalogues of desmosterol, cholesterol, 7-dehydrocholesterol by thin-layer and gas-liquid chromatography]
- PMID: 1208403
[Separate measurement of fluoroanalogues of desmosterol, cholesterol, 7-dehydrocholesterol by thin-layer and gas-liquid chromatography]
Abstract
An attempt was made to develop a new method for separate measurement of sterols of similar chemical structure--desmosterol, cholesterol, 7-dehydrocholesterol--by means of thin-layer and gas-liquid chromatography. Fluoroanalogues of the sterols were well separated in the adsorbent thin layer. This facilitated their further identification, quantitation and accumulation. Similar results were obtained with the aid of gas liquid chromatography.