Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Jun;27(3):261-72.
doi: 10.1007/BF02704915.

Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

T Venugopal  1 , S Mathavan, T J Pandian
Affiliations

Molecular cloning of growth hormone encoding cDNA of Indian major carps by a modified rapid amplification of cDNA ends strategy

T Venugopal et al. J Biosci. 2002 Jun.

Abstract

A modified rapid amplification of cDNA ends (RACE) strategy has been developed for cloning highly conserved cDNA sequences. Using this modified method, the growth hormone (GH) encoding cDNA sequences of Labeo rohita, Cirrhina mrigala and Catla catla have been cloned, characterized and overexpressed in Escherichia coli. These sequences show 96-98% homology to each other and are about 85% homologous to that of common carp. Besides, an attempt has been made for the first time to describe a 3-D model of the fish GH protein.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1982 Feb;2(2):161-70 - PubMed
    1. Gen Comp Endocrinol. 1991 Oct;84(1):135-46 - PubMed
    1. Nucleic Acids Res. 1996 Feb 15;24(4):795-6 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. Trends Genet. 1993 Mar;9(3):70-1 - PubMed

Publication types

LinkOut - more resources