A method of reversible inactivation of small regions of brain tissue

Abstract

A method is described for reversibly inactivating small, precisely localized regions of brain tissue with injections of nanoliter quantities of the local anesthetic lidocaine hydrochloride. The injections are made through a combined recording-injection probe consisting of a glass micropipette onto whose outer surface is plated a metallic cylinder for recording extracellular action potentials. The recording cylinder for recording extracellular action potentials. The recording cylinder, located a known distance from the pipette tip, picks up a continuous, large-amplitude, multiunit response which can be used to accurately position the tip according to physiological criteria. It also provides a means of determining the duration of the anesthetic block and estimating its spreat. This devise has been used to selectively block 200--400 micrometers regions of individual laminae of the lateral geniculate nucleus centered within 50 micrometers of a retinotopically defined target site. The blocks last from 3 to 10 min and can be repeated many times at the same location.