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. 2002 Jul;9(4):935-7.
doi: 10.1128/cdli.9.4.935-937.2002.

Interleukin-8 and tumor necrosis factor alpha production in human epidermal keratinocytes induced by Trichophyton mentagrophytes

Yuka Nakamura  1 , Rui Kano, Atsuhiko Hasegawa, Shinichi Watanabe
Affiliations

Interleukin-8 and tumor necrosis factor alpha production in human epidermal keratinocytes induced by Trichophyton mentagrophytes

Yuka Nakamura et al. Clin Diagn Lab Immunol. 2002 Jul.

Abstract

Production of interleukin-8 (IL-8) and tumor necrosis factor alpha (TNF-alpha) was confirmed by enzyme-linked immunosorbent assay in a medium where human epidermal keratinocytes were cocultured with Trichophyton mentagrophytes for 1 to 12 h. IL-8 and TNF-alpha mRNAs were also detected in the keratinocytes cocultured with T. mentagrophytes.

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Figures

FIG. 1.
FIG. 1.
After 1, 3, 6, and 12 h of coculture with the microconidia and keratinocytes, cytokine levels in the supernatants were measured by the ELISA method. IL-8 and TNF-α levels in the supernatant were increased following coculture. The statistical significance was determined by the nonpaired t test.
FIG. 2.
FIG. 2.
The mRNA of IL-8 and TNF-α in human epidermal keratinocytes cocultured with T. mentagrophytes. After 0, 1, 3, and 12 h of coculture with human epidermal keratinocytes and T. mentagrophytes, mRNAs of IL-8 and TNF-α in the keratinocytes were determined by RT-PCR. IL-8 and TNF-α of mRNAs were detected after 1 to 12 h of coculture but not at 0 h of coculture.
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