[Characteristics of poly(A) rich RNA in human lymphocytes under normal conditions and in chronic lympholeukemia]

Abstract

The presence of poly(A) containing RNA bound specifically to millipore filters, adsorbed on poly(U) cellulose and resistant to the pancreatic RNAs effect has been demonstrated in total RNA of human lymphocytes. These fractions show a DNA-like nucleotid content, a rapid turnover and they are characterized by sedimentation constants within the range of 9.5--15 S. On the average, 24.6 per cent of the applied initial radioactivity of total RNA is absorbed on membrane filters of normal lymphocytes, whereas from 13.3 to 23.3 per cent- in the poly(U) cellulose column. The study of lymphocytes total RNA in patients with chronic lympholeucosis has indicated a great heterogeneity in the content of poly(A) sequences rich RNA fractions.