Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2002 Jul;161(1):7-11.
doi: 10.1016/S0002-9440(10)64150-8.

Molecular profiling of angiogenic markers: a step towards interpretive analysis of a complex biological function

Kevin P Claffey  1
Affiliations
Comment

Molecular profiling of angiogenic markers: a step towards interpretive analysis of a complex biological function

Kevin P Claffey. Am J Pathol. 2002 Jul.
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Model of tumor angiogenic stages in relation to therapeutic intervention. Representation of the angiogenic stages in the initiation and expansion of tumor as well as the possible result for therapeutic intervention. Text indicates angiogenic events, cell types, and changes in microenvironment for each stage. Transitions between stages are indicated as 1: active and proliferative angiogenic response, 2: maturation into functional tumor vasculature, 3: effective anti-angiogenic therapy to derive a balance between tumor support and apoptosis, 4: re-entry of treated tumor into active angiogenesis due to increased expression of angiogenic stimuli from affected tumor or therapeutic relapse, 5: anti-angiogenic therapy alone that will benefit from self-reactivity to stable disease, 6: effective multi-modal therapeutics effective for blocking both angiogenic and tumor growth events, 7: long-term relapse from a small number of therapeutically resistant or static cells selected for survival.
Figure 2.
Figure 2.
Diagnostic three-dimensional functional MRI identification of angiogenic prostate tumor in mice. Three mice were scanned without contrast agent in a 1.5 Tesla MR using a peripheral coil across the lower abdomen for development of orthotopically injected PC-3 prostate tumors. Functional MRI parameters were computed for the inverse transition rate (R2′) and the images aligned and reconstructed in 3-D. Animal on right indicates a tumor with prominent R2′ signal (gray, arrow) on the periphery of a defined tumor later confirmed by dissection and histological analysis. Note the differential levels of gray signal ranging from light to dark foci. The normal tissues appear as mosaic white/light gray.
Figure 3.
Figure 3.
A multiplex approach to profiling the complex angiogenic process. Analytical and quantitative profiling methodologies are defined on the spokes around the central angiogenic processes. Given the variability in physiological and pathophysiological angiogenic activators, some of which are indicated around the central process, the analysis of the angiogenic responses in each condition may define specific molecular profiles for each.
See this image and copyright information in PMC

Comment on

  • Molecular profiling of angiogenesis markers.
    Shih SC, Robinson GS, Perruzzi CA, Calvo A, Desai K, Green JE, Ali IU, Smith LE, Senger DR. Shih SC, et al. Am J Pathol. 2002 Jul;161(1):35-41. doi: 10.1016/S0002-9440(10)64154-5. Am J Pathol. 2002. PMID: 12107087 Free PMC article.

References

    1. Shih S-C, Robinson GS, Peruzzi C, Smith LE, Senger D: Molecular profiling of angiogenic markers. Am J Pathol 2002, 161:35-41 - PMC - PubMed
    1. Folkman J, Haudenschild C: Angiogenesis by capillary endothelial cells in culture. Trans Ophthalmol Soc U K 1980, 100:346-353 - PubMed
    1. Kubota Y, Kleinman HK, Martin GR, Lawley TJ: Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J Cell Biol 1988, 107:1589-1598 - PMC - PubMed
    1. Gamble JR, Matthias LJ, Meyer G, Kaur P, Russ G, Faull R, Berndt MC, Vadas MA: Regulation of in vitro capillary tube formation by anti-integrin antibodies. J Cell Biol 1993, 121:931-943 - PMC - PubMed
    1. Teichert-Kuliszewska K, Maisonpierre PC, Jones N, Campbell AI, Master Z, Bendeck MP, Alitalo K, Dumont DJ, Yancopoulos GD, Stewart DJ: Biological action of angiopoietin-2 in a fibrin matrix model of angiogenesis is associated with activation of Tie-2. Cardiovasc Res 2001, 49:659-670 - PubMed

Publication types

MeSH terms

LinkOut - more resources