Nuclear localization of CDC25B1 and serine 146 integrity are required for induction of mitosis
- PMID: 12107172
- DOI: 10.1074/jbc.M204430200
Nuclear localization of CDC25B1 and serine 146 integrity are required for induction of mitosis
Abstract
CDC25B phosphatases are essential regulators that control cyclin-dependent kinases activities at the entry into mitosis. In this study, we demonstrate that serine 146 is required for two crucial features of CDC25B1. It is essential for CDC25B1 to function as a mitotic inducer and to prevent CDC25B1 export from the nucleus. We also show that serine 146 is phosphorylated in vitro by CDK1-cyclin B. However, phosphorylation of CDC25B does not stimulate its phosphatase activity, and mutation of serine 146 had no effect on its catalytic activity. Serine 146 phosphorylation is proposed to be a key event in the regulation of the CDC25B function in the initiation of mammalian mitosis.
Similar articles
-
CDC25B phosphorylation by Aurora-A occurs at the G2/M transition and is inhibited by DNA damage.Cell Cycle. 2005 Sep;4(9):1233-8. doi: 10.4161/cc.4.9.1964. Epub 2005 Sep 23. Cell Cycle. 2005. PMID: 16082213
-
The polo-like kinase 1 regulates CDC25B-dependent mitosis entry.Biochim Biophys Acta. 2009 Mar;1793(3):462-8. doi: 10.1016/j.bbamcr.2008.12.015. Epub 2009 Jan 2. Biochim Biophys Acta. 2009. PMID: 19185590
-
Phosphorylation of CDC25B by Aurora-A at the centrosome contributes to the G2-M transition.J Cell Sci. 2004 May 15;117(Pt 12):2523-31. doi: 10.1242/jcs.01108. Epub 2004 May 5. J Cell Sci. 2004. PMID: 15128871
-
Control of mitosis by changes in the subcellular location of cyclin-B1-Cdk1 and Cdc25C.Curr Opin Cell Biol. 2000 Dec;12(6):658-65. doi: 10.1016/s0955-0674(00)00149-6. Curr Opin Cell Biol. 2000. PMID: 11063929 Review.
-
Cell cycle regulation by the Cdc25 phosphatase family.Prog Cell Cycle Res. 2000;4:107-14. doi: 10.1007/978-1-4615-4253-7_10. Prog Cell Cycle Res. 2000. PMID: 10740819 Review.
Cited by
-
Prognostic significance of CDC25B expression in gliomas.J Clin Pathol. 2006 Jul;59(7):725-8. doi: 10.1136/jcp.2005.029546. J Clin Pathol. 2006. PMID: 16803948 Free PMC article.
-
Chk1 Activation Protects Rad9A from Degradation as Part of a Positive Feedback Loop during Checkpoint Signalling.PLoS One. 2015 Dec 11;10(12):e0144434. doi: 10.1371/journal.pone.0144434. eCollection 2015. PLoS One. 2015. PMID: 26658951 Free PMC article.
-
Pathways for genome integrity in G2 phase of the cell cycle.Biomolecules. 2012 Nov 30;2(4):579-607. doi: 10.3390/biom2040579. Biomolecules. 2012. PMID: 24970150 Free PMC article.
-
CDC25A phosphatase controls meiosis I progression in mouse oocytes.Dev Biol. 2008 May 1;317(1):260-9. doi: 10.1016/j.ydbio.2008.02.028. Epub 2008 Mar 4. Dev Biol. 2008. PMID: 18367163 Free PMC article.
-
The decision to enter mitosis: feedback and redundancy in the mitotic entry network.J Cell Biol. 2009 Apr 20;185(2):193-202. doi: 10.1083/jcb.200812045. Epub 2009 Apr 13. J Cell Biol. 2009. PMID: 19364923 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous