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. 1979 Nov;26(2):585-9.
doi: 10.1128/iai.26.2.585-589.1979.

Natural phosphorylation of group A streptococcal pyrogenic exotoxin type C

Natural phosphorylation of group A streptococcal pyrogenic exotoxin type C

P M Schlievert et al. Infect Immun. 1979 Nov.

Abstract

Group A streptococcal pyrogenic exotoxin (SPE) type C, produced by strain T18P grown in the presence of 32P, was separated from culture supernatant fluids by using alcohol precipitation. The resulting toxin (EtOH-1) contained 3 X 10(6) to 5 X 10(6) cpm of 32P per milligram of protein. The radiolabel migrated with SPE C during isoelectric focusing in polyacrylamide gels (pI 6.7) and double immunodiffusion, in which the toxin formed a line of identity with highly purified SPE C when reacted with hyperimmune antisera raised against SPE C. The EtOH-1 radiolabeled toxin was pyrogenic and had the capacity to enhance host susceptibility to lethal endotoxin shock. EtOH-1 toxin lost both radiolabel and biological activity after being treated with alkaline phosphatase. The nonspecific lymphocyte mitogenicity of purified unlabeled SPE C was stimulated by adenosine monophosphate but not adenosine, adenosine diphosphate, or adenosine triphosphate. Adenosine monophosphate may function as a cofactor of SPE C and contribute the phosphate group required for biological activity.

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References

    1. J Infect Dis. 1955 Jan-Feb;96(1):14-8 - PubMed
    1. Infect Immun. 1979 Nov;26(2):467-72 - PubMed
    1. Infect Immun. 1977 May;16(2):673-9 - PubMed
    1. Infect Immun. 1978 Jan;19(1):51-7 - PubMed
    1. Infect Immun. 1978 Feb;19(2):470-6 - PubMed

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