doi: 10.1016/s0021-9673(01)95313-0.
Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography
- PMID: 121120
- DOI: 10.1016/s0021-9673(01)95313-0
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Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography
J Chromatogr.
.
Abstract
A sensitive method for the determination of chondroitin 4- and 6-sulphate is presented. After chondroitinase digestion of the chondroitin sulphate preparations, the obtained disaccharides are separated on a weak anion-exchange resin in a high-performance liquid chromatography system. The method was used to study 4-sulphate to 6-sulphate ratios in chondroitin sulphates prepared from bovine nasal cartilage and human nucleus pulposus. The results show clearly that these two preparations contain considerable amounts of both isomers.
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