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. 1979 Apr 1:171:339-44.
doi: 10.1016/s0021-9673(01)95313-0.

Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography

A Hjerpe, C A Antonopoulos, B Engfeldt

Determination of sulphated disaccharides from chondroitin sulphates by high-performance liquid chromatography

A Hjerpe et al. J Chromatogr. .

Abstract

A sensitive method for the determination of chondroitin 4- and 6-sulphate is presented. After chondroitinase digestion of the chondroitin sulphate preparations, the obtained disaccharides are separated on a weak anion-exchange resin in a high-performance liquid chromatography system. The method was used to study 4-sulphate to 6-sulphate ratios in chondroitin sulphates prepared from bovine nasal cartilage and human nucleus pulposus. The results show clearly that these two preparations contain considerable amounts of both isomers.

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