Application of size-exclusion chromatography-inductively coupled plasma mass spectrometry for fractionation of element species in seeds of legumes
- PMID: 12113984
- DOI: 10.1016/s1570-0232(02)00296-9
Application of size-exclusion chromatography-inductively coupled plasma mass spectrometry for fractionation of element species in seeds of legumes
Abstract
Fractionation of soluble species of P, Mn, Fe, Co, Ni, Cu, Zn, Se and Mo in pea and lentil seeds was made by on-line hyphenation of size-exclusion chromatography (SEC) and inductively coupled plasma mass spectrometry. Seed samples were extracted with 0.02 mol l(-1) Tris-HCl buffer solution, pH 7.5. SEC was performed on Superdex 75 and Superdex Peptide columns (300 x 10 mm) with the same buffer solution as the mobile phase. Monitoring of oxide ion 47(PO)+ was used for detection of phosphorus compounds. Other elements were detected as ions of 55Mn, 57Fe, 59Co, 62Ni, 65Cu, 66Zn, 82Se and 95Mo nuclides. Elements in individual elution zones were quantified using external calibration. Complete chromatographic recoveries of elements were found in cases of phosphorus, nickel and copper. Substantial parts of manganese and zinc, as well as traces of cobalt, selenium and molybdenum are retained on the column. Injection of EDTA solution removes these elements from the column. Chromatographic profiles of pea and lentil samples are very similar for all elements except Mo. Main element species in the high-molecular-mass region (approx. 190,000 rel. mol. mass unit) were detected in case of Fe. Low-molecular-mass species (<2000 rel. mol. mass unit) as major element forms are typical for Cu and Zn.
Copyright 2002 Elsevier Science BV.
Similar articles
-
Fractionation of phosphorus and trace elements species in soybean flour and common white bean seeds by size exclusion chromatography-inductively coupled plasma mass spectrometry.J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Apr 25;770(1-2):261-73. doi: 10.1016/s1570-0232(02)00053-3. J Chromatogr B Analyt Technol Biomed Life Sci. 2002. PMID: 12013235
-
Studies of various elements of nutritional and toxicological interest associated with different molecular weight fractions in Brazil nuts.J Agric Food Chem. 2004 Sep 22;52(19):5773-80. doi: 10.1021/jf0496649. J Agric Food Chem. 2004. PMID: 15366819
-
Distribution of elements binding to molecules with different molecular weights in aqueous extract of Antarctic krill by size-exclusion chromatography coupled with inductively coupled plasma mass spectrometry.J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 5;814(1):83-91. doi: 10.1016/j.jchromb.2004.10.008. J Chromatogr B Analyt Technol Biomed Life Sci. 2005. PMID: 15607711
-
Quantification of copper and zinc species fractions in legume seeds extracts by SEC/ICP-MS: validation and uncertainty estimation.Talanta. 2002 Jul 19;57(6):1133-42. doi: 10.1016/s0039-9140(02)00151-0. Talanta. 2002. PMID: 18968719
-
Measurement of trace elements in proteins extracted from liver by size exclusion chromatography-inductively coupled plasma-mass spectrometry with a magnetic sector mass spectrometer.Anal Biochem. 2001 Jan 1;288(1):89-96. doi: 10.1006/abio.2000.4885. Anal Biochem. 2001. PMID: 11141310
Cited by
-
Influence of Dietary Fibre and Protein Fractions on the Trace Element Bioaccessibility of Turnip Tops (Brassica rapa) Growing under Mediterranean Conditions.Foods. 2024 Feb 1;13(3):462. doi: 10.3390/foods13030462. Foods. 2024. PMID: 38338598 Free PMC article.
-
In Vitro Assessment of the Physiologically Relevant Oral Bioaccessibility of Metallic Elements in Edible Herbs Using the Unified Bioaccessibility Protocol.Molecules. 2023 Jul 14;28(14):5396. doi: 10.3390/molecules28145396. Molecules. 2023. PMID: 37513269 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources