Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2002 Aug;46(8):2720-2.
doi: 10.1128/AAC.46.8.2720-2722.2002.

Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

Juan-Carlos Palomino  1 , Anandi Martin, Mirtha Camacho, Humberto Guerra, Jean Swings, Françoise Portaels
Affiliations

Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis

Juan-Carlos Palomino et al. Antimicrob Agents Chemother. 2002 Aug.

Abstract

A method for detecting multidrug-resistant Mycobacterium tuberculosis by using a reduction of resazurin is described. Eighty clinical isolates were evaluated against isoniazid and rifampin; results at 7 days were compared with those of the proportion method. Specificity and sensitivity were excellent. The method is simple, inexpensive, and rapid and might be used with other antituberculosis drugs.

PubMed Disclaimer

References

    1. Canetti, G., S. Froman, J. Grosset, P. Hauduroy, M. Langerova, H. T. Mahler, G. Meissner, D. A. Mitchison, and L. Sula. 1963. Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull. W. H. O. 29:565-578. - PMC - PubMed
    1. Canetti, G., W. Fox, A. Khomenko, H. T. Mahler, N. K. Menon, D. A. Mitchison, N. Rist, and N. A. Smelev. 1969. Advances in techniques of testing mycobacterial drug sensitivity and the use of sensitivity tests in tuberculosis control programs. Bull. W. H. O. 41:21-43. - PMC - PubMed
    1. Carter, R. A., S. A. Ericsson, C. D. Corn, P. R. Weyerts, M. G. Dart, S. G. Escue, and J. Mesta. 1998. Assessing the fertility potential of equine semen samples using the reducible dyes methylene green and resazurin. Arch. Androl. 40:59-66. - PubMed
    1. Coates, A. R., and D. A. Mitchison. 1983. The role of sensitivity tests in short-term chemotherapy. Bull. Int. Union Tuberc. 58:111-114. - PubMed
    1. Collins, L., and S. G. Franzblau. 1997. Microplate Alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob. Agents Chemother. 41:1004-1009. - PMC - PubMed

Publication types

LinkOut - more resources