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. 1975 Oct;151(1):145-8.
doi: 10.1042/bj1510145.

The purification and properties of a beta-N-acetylhexosaminidase from Trichomonas foetus

R G Edwards, P Thomas, J H Westwood

The purification and properties of a beta-N-acetylhexosaminidase from Trichomonas foetus

R G Edwards et al. Biochem J. 1975 Oct.

Abstract

A beta-N-acetylhexosaminidase was purified 800-fold from extracts of Trichomonas foetus by affinity chromatography on a column of N-(epsilon-aminohexanoyl)-2-acetamido-2-deoxy-beta-D-glucopyranosylamine bound to CNBr-activated Sepharose. The enzyme has a dual specificity for the p-nitrophenyl beta-D-glycosides of N-acetylglucosamine and N-acetyl-galactosamine. The parent sugars are both competitive inhibitors. The enzyme has a mol. wt. approx. 150000 and a pH optimum of 6.2. It is suggested that the same active site catalyses both activities and that no part is played by the 4-hydroxyl group in substrate binding, but it is involved in determining the catalytic rate.

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