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Comparative Study
. 1979 Oct;43(4):420-5.

The bovine immune response to Brucella abortus. II. Elimination of some sporadic serological reactions by chelation of divalent cations

K Nielsen, B S Samagh, G Speckmann, B Stemshorn
Comparative Study

The bovine immune response to Brucella abortus. II. Elimination of some sporadic serological reactions by chelation of divalent cations

K Nielsen et al. Can J Comp Med. 1979 Oct.

Abstract

The standard agglutination tests for detecting antibody to Brucella abortus were modified by addition of chelating agents (EDTA and EGTA) to the antigens. Approximately 80% of "singleton" agglutination test reactions, negative on the diagnostic complement fixation test, obtained with cattle sera were eliminated while no decrease in titer was apparent when sera from B. abortus infected or vaccinated cattle were tested.

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References

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