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. 1975 Dec;55(4):503-14.
doi: 10.1111/j.1476-5381.1975.tb07425.x.

The development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods

K K Dighe, H A Emslie, L K Henderson, L Simon

The development of antisera to prostaglandins B2 and F2alpha and their analysis using solid-phase and double antibody radioimmunoassay methods

K K Dighe et al. Br J Pharmacol. 1975 Dec.

Abstract

1 A method for the production of highly substituted prostaglandin-bovine serum albumin conjugates has been developed. 2 Antisera to prostaglandins B2 and F2alpha were raised in rabbits immunized with prostaglandin-bovine serum albumin conjugates. 3 The antisera were assessed for specificity and sensitivity by the double antibody radioimmunoassay method and after they were covalently linked to powdered cellulose to form a 'solid-phase' system. 4 Solid phase radioimmunoassays were developed using conventional shaking and in the presence of sucrose which obviates the need for continuous mixing of the incubates.

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