doi: 10.2174/0929866023409057.
Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system
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- PMID: 12141922
- DOI: 10.2174/0929866023409057
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Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system
Protein Pept Lett.
2002 Feb.
Abstract
A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.
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