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. 2002 Feb;9(1):39-43.
doi: 10.2174/0929866023409057.

Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system

Beatriz dos Santos Ferreira  1 , Alan Trindade Branco, Karlla Salim de Queiroz, Gonçalo Apolinàrio de Souza Filho
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Two-dimensional protein analysis by isoelectric focusing in a multi-cell plate system

Beatriz dos Santos Ferreira et al. Protein Pept Lett. 2002 Feb.

Abstract

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

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