Combined classical and molecular cytogenetic analysis of cancer
- PMID: 12142045
- DOI: 10.1016/s0959-8049(02)00117-x
Combined classical and molecular cytogenetic analysis of cancer
Abstract
While chromosome-banding analysis has set the standard for karyotyping from 1970 onwards, fluorescent in situ hybridisation (FISH) has more recently been used to complement the study of chromosomal rearrangements. Especially useful has been the appearance of FISH methodologies with screening abilities, namely comparative genome hybridisation (CGH), multicolour-FISH (m-FISH), and cross-species colour banding (RxFISH). These FISH-based screening techniques are reviewed here together with methodologies using chromosome- or locus-specific probes. Emphasis is put on the strengths and limitations of these FISH techniques to complement standard chromosome banding analysis. Judicious choice from the molecular cytogenetic techniques now available has significantly improved our ability to characterise the genomic rearrangements of cancer cells.
Similar articles
-
Combined RxFISH/G-banding allows refined karyotyping of solid tumors.Hum Genet. 1999 May;104(5):370-5. doi: 10.1007/s004390050970. Hum Genet. 1999. PMID: 10394927
-
[Strategies to identify supernumerary chromosomal markers in constitutional cytogenetics].Pathol Biol (Paris). 2008 Sep;56(6):362-7. doi: 10.1016/j.patbio.2008.03.012. Epub 2008 May 5. Pathol Biol (Paris). 2008. PMID: 18456432 Review. French.
-
Complete cytogenetic characterization of the human breast cancer cell line MA11 combining G-banding, comparative genomic hybridization, multicolor fluorescence in situ hybridization, RxFISH, and chromosome-specific painting.Cancer Genet Cytogenet. 2001 Nov;131(1):25-30. doi: 10.1016/s0165-4608(01)00484-8. Cancer Genet Cytogenet. 2001. PMID: 11734314
-
Detailed genome-wide screening for inter- and intrachromosomal abnormalities by sequential G-banding and RxFISH color banding of the same metaphase cells.Cancer Genet Cytogenet. 2000 Jun;119(2):94-101. doi: 10.1016/s0165-4608(99)00220-4. Cancer Genet Cytogenet. 2000. PMID: 10867142
-
Multicolor chromosome bar codes.Cytogenet Genome Res. 2006;114(3-4):245-9. doi: 10.1159/000094208. Cytogenet Genome Res. 2006. PMID: 16954661 Review.
Cited by
-
Methylation-specific multiplex ligation-dependent probe amplification in meningiomas.J Neurooncol. 2008 Dec;90(3):267-73. doi: 10.1007/s11060-008-9672-8. Epub 2008 Sep 2. J Neurooncol. 2008. PMID: 18763019
-
[Comparative genomic hybridization as cytogenetic tumor diagnosis technique in juvenile angiofibroma].HNO. 2003 Dec;51(12):959-61. doi: 10.1007/s00106-003-0934-7. HNO. 2003. PMID: 14647923 German. No abstract available.
-
Methylation-specific multiplex ligation-dependent probe amplification and its impact on clinical findings in medulloblastoma.J Neurooncol. 2014 Jan;116(2):213-20. doi: 10.1007/s11060-013-1286-0. Epub 2013 Oct 27. J Neurooncol. 2014. PMID: 24162828
-
Cytogenetic risk groups for childhood acute myeloid leukemia based on survival analysis in a cancer referral hospital from Perú.Biomedica. 2021 Jun 29;41(2):302-313. doi: 10.7705/biomedica.5747. Biomedica. 2021. PMID: 34214271 Free PMC article. English, Spanish.
-
Diagnostic molecular pathology: current techniques and clinical applications, part I.Proc (Bayl Univ Med Cent). 2003 Oct;16(4):379-83. doi: 10.1080/08998280.2003.11927931. Proc (Bayl Univ Med Cent). 2003. PMID: 16278751 Free PMC article. No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
