Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1975 Dec 30;162(1):23-7.
doi: 10.1007/BF02123574.

Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses

K Tobita
Comparative Study

Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B viruses

K Tobita. Med Microbiol Immunol. .

Abstract

A wide range of influenza B virus strains with various passage histories uniformly formed well-defined clear plaques with high efficiency in cultures of an established line of canine kidney cells (MDCK). PFU titers of the viruses assayed in MDCK exceeded the titers assayed in ovo. With recently isolated strains such as B/Hong Kong/5/72 and Gifu/2/73, the PFU/EID50 ratios were as high as 100 to 400. MDCK cells have been successfully employed for primary isolation of influenza B viruses from throat washings of patients by direct plaquing.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Appl Microbiol. 1968 Apr;16(4):588-94 - PubMed
    1. J Gen Virol. 1968 Sep;3(2):285-7 - PubMed
    1. Arch Gesamte Virusforsch. 1967;20(2):278-82 - PubMed
    1. J Virol. 1974 Feb;13(2):347-52 - PubMed
    1. J Gen Virol. 1974 Mar;22(3):347-54 - PubMed

Publication types

LinkOut - more resources