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. 2002 Aug;68(8):3965-8.
doi: 10.1128/AEM.68.8.3965-3968.2002.

Characterization of virus adsorption by using DEAE-sepharose and octyl-sepharoser

Patricia A Shields  1 , Samuel R Farrah
Affiliations

Characterization of virus adsorption by using DEAE-sepharose and octyl-sepharoser

Patricia A Shields et al. Appl Environ Microbiol. 2002 Aug.

Abstract

Viruses were characterized by their adsorption to DEAE-Sepharose or by their elution from octyl-Sepharose by using buffered solutions of sodium chloride with different ionic strengths. Viruses whose adsorption to DEAE-Sepharose was reduced most rapidly by an increase in the sodium chloride concentration were considered to have the weakest electrostatic interactions with the solids; these viruses included MS2, E1, and phiX174. Viruses whose adsorption to DEAE-Sepharose was reduced least rapidly were considered to have the strongest electrostatic interactions with the column; these viruses included P1, T4, T2, and E5. All of the viruses studied adsorbed to octyl-Sepharose in the presence of 4 M NaCl. Viruses that were eluted most rapidly following a decrease in the concentration of NaCl were considered to have the weakest hydrophobic interactions with the column; these viruses included phiX174, CB4, and E1. Viruses that were eluted least rapidly from the columns after the NaCl concentration was decreased were considered to have the strongest hydrophobic interactions with the column; these viruses included f2, MS2, and E5.

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Figures

FIG. 1.
FIG. 1.
Adsorption of T2 and echovirus 1 to DEAE-Sepharose as a function of the ionic strengths of solutions at pH 7.0.
FIG. 2.
FIG. 2.
Elution of echovirus 1 and T3 adsorbed to octyl-Sepharose as a function of the ionic strengths of eluting solutions at pH 7.
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References

    1. Bales, R. C., S. Li, K. M. Maguire, M. T. Yahya, and C. P. Gerba. 1993. MS-2 and poliovirus transport in porous media: hydrophobic effects and chemical perturbations. Water Resour. Res. 29:957-963.
    1. Bitton, G. 1975. Adsorption of viruses onto surfaces in soil and water. Water Res. 9:473-484.
    1. Cantor, C. R., and P. R. Schimmel. 1980. Biophysical chemistry, p. 676-678. W. H. Freeman & Co., San Francisco, Calif.
    1. Dizer, H., A. Nasser, and J. M. Lopez. 1984. Penetration of different human pathogenic viruses into sand columns percolated with distilled water, groundwater, or wastewater. Appl. Environ. Microbiol. 47:409-415. - PMC - PubMed
    1. Dowd, S. E., S. D. Pillai, S. Wang, and M. Y. Corapcioglu. 1998. Delineating the specific influence of virus isoelectric point and size on virus adsorption and transport through sandy soils. Appl. Environ. Microbiol. 64:405-410. - PMC - PubMed

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