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. 2002 Aug 7;124(31):9026-7.
doi: 10.1021/ja027007w.

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli

Jason W Chin  1 , Stephen W Santoro, Andrew B Martin, David S King, Lei Wang, Peter G Schultz
Affiliations

Addition of p-azido-L-phenylalanine to the genetic code of Escherichia coli

Jason W Chin et al. J Am Chem Soc. .

Abstract

We report the selection of a new orthogonal aminoacyl tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in response to the amber codon, TAG. The amino acid is incorporated in good yield with high fidelity and can be used to crosslink interacting proteins.

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