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. 2002 Aug;40(8):3035-7.
doi: 10.1128/JCM.40.8.3035-3037.2002.

Isolation from a healthy carrier and characterization of a Neisseria meningitidis strain that is deficient in gamma-Glutamyl aminopeptidase activity

Hideyuki Takahashi  1 , Hiroshi Tanaka, Hiroo Inouye, Toshiro Kuroki, Yuko Watanabe, Shiro Yamai, Haruo Watanabe
Affiliations

Isolation from a healthy carrier and characterization of a Neisseria meningitidis strain that is deficient in gamma-Glutamyl aminopeptidase activity

Hideyuki Takahashi et al. J Clin Microbiol. 2002 Aug.

Abstract

gamma-Glutamyl aminopeptidase (GGT) activity is used as a specific marker for the identification of Neisseria meningitidis. We isolated from a healthy carrier and characterized an N. meningitidis isolate which lacked the activity due to the insertional mutation of the ggt gene, suggesting that naturally occurring N. meningitidis isolates do not always possess GGT activity.

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Figures

FIG. 1.
FIG. 1.
PCR amplification of the ggt gene from NIID113 (lane 2), H44/76 (lane 3), and other N. meningitidis clinical isolates, namely, NIID57 (lane 4), NIID99 (lane 5), and NIID100 (lane 6). Molecular mass markers are shown in lane 1.
FIG. 2.
FIG. 2.
Insertional site of IS in ggt. The vertical arrow behind the T residue (position 1165) indicates the insertional site of the IS. The IS sequence is shown in italics. Amino acid residues shaded in gray indicate the additional amino acid residues derived from the insertional sequence. An asterisk indicates the stop codon.
See this image and copyright information in PMC

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