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. 2002 Aug;40(8):3066-7.
doi: 10.1128/JCM.40.8.3066-3067.2002.

Detection of enteroaggregative Escherichia coli with formalin-preserved HEp-2 cells

Mohamad S Miqdady  1 , Zhi-Dong Jiang, James P Nataro, Herbert L DuPont
Affiliations

Detection of enteroaggregative Escherichia coli with formalin-preserved HEp-2 cells

Mohamad S Miqdady et al. J Clin Microbiol. 2002 Aug.

Abstract

Formalin-stored HEp-2 cells were used to assay Escherichia coli for adherence. Cells refrigerated in formalin for up to 28 days and used in a wet assay format demonstrated an assay sensitivity ranging from 94 to 98% to detect enteroaggregative E. coli (EAEC). HEp-2 cells first fixed and stored with formalin and then stored dry in ambient conditions for 6 weeks demonstrated an assay sensitivity of 92% to detect EAEC. Using formalin-fixed HEp-2 cells will improve the efficiency of EAEC identification.

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