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. 2002 Aug;40(8):3082-5.
doi: 10.1128/JCM.40.8.3082-3085.2002.

Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients

Eun-jeong Heo  1 , Jin-ho Park, Ja-ryong Koo, Man-suk Park, Mi-yeoun Park, J Stephen Dumler, Joon-seok Chae
Affiliations

Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients

Eun-jeong Heo et al. J Clin Microbiol. 2002 Aug.

Abstract

Sera from 491 Korean patients with acute febrile diseases were tested for Ehrlichia chaffeensis and Anaplasma phagocytophila antibodies by indirect immunofluorescence assay (IFA), Western blotting, and TaqMan real-time PCR. Overall, 0.4% of sera reacted with E. chaffeensis, and 1.8% reacted with A. phagocytophila in IFAs. This is the first report of detection of antibodies to A. phagocytophila and E. chaffeensis in Korea and suggests the presence of A. phagocytophila and E. chaffeensis or antigenically similar species.

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Figures

FIG. 1.
FIG. 1.
Western blot with A. phagocytophila (A) and E. chaffeensis (B) as antigens reacted with IFA-positive serum of Korean patients with acute fever. (A) Lanes: M, size marker; 1, uninfected HL-60 cell antigen reacted with A. phagocytophila IFA-negative serum; 2, uninfected HL-60 cell antigen reacted with A. phagocytophila IFA-positive serum; 3, purified A. phagocytophila antigens reacted with monoclonal antibody 20B4, which detects A. phagocytophila Msp2 (44-kDa protein antigen; 4, purified A. phagocytophila antigens reacted with A. phagocytophila IFA-positive Korean human serum. (B) Lanes: M, size marker; 1, DH82 cell antigens reacted with E. chaffeensis IFA-negative serum; 2, DH82 cell antigens reacted with E. chaffeensis IFA-positive control serum from a U.S. patient; 3, purified E. chaffeensis antigen reacted with E. chaffeensis IFA-positive control serum from a U.S. patient; 4 to 5, purified E. chaffeensis antigen reacted with E. chaffeensis IFA-positive Korean human sera.
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