[Enzymic determination of total serum cholesterol with centrifugal analyzers (author's transl)]

Abstract

The enzymic determination of total cholesterol described by P. Röschlau (Z. Klin. Chem. Klin. Biochem. 12, 403-407 (1974)) was modified and adapted to the Centrifichem system. The method which requires only 5 mul serum, is accurate, quick and simple. A separate serum blank is not necessary. Compared with the manual method, smaller quantities of reagents used and the resulting decrease in cost are appreciable for large series of analyses. The course of the reaction at 30 and 37 degrees C, the linearity, calibration, precision, recovery, and correlation with the Lieberman-Burchard method and the enzymic manual method are described.